In-Vitro Conservation of Tylophora Indica: A Threatened Medicinal Plant
The IUP Journal of Genetics & Evolution, Vol. II, No. 3, pp. 26-35, August 2009
Posted: 3 Aug 2009
Date Written: August 3, 2009
Abstract
A protocol for in-vitro conservation of Tylophora indica (Burm. f.) Merrill - an important medicinal plant -has been developed. T. indica plants cultured in MS medium supplemented with 0.54 μM NAA and 4.44 μM BAP produced 5-10 shoots within a period of two months. For in vitro conservation studies, experiments were carried out in 2-3 week maintained vitroplants under Standard and Reduced Culture Conditions (SCC and RCC). Vitroplants could be successfully conserved in Full Strength MS Medium (FMS) under SCC for six months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature regimen (15 oC and 10 oC) and reduction in media constituents do not appear to favor conservation, although the former accomplished conservation levels equal to FMS under SCC.
Keywords: In-vitro conservation, Low temperature, Standard Culture Conditions (SCC), Reduced Culture Conditions (RCC), Full Strength MS Medium (FMS)
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