The Implications of Tidiazuron (TDZ) in the Induction of Callus and Plant Regeneration in the Dianthus Spiculifolius Schur. Variety
Analele Universităţii din Oradea, Fascicula Protecţia Mediului - Vol. XXI, 2013
8 Pages Posted: 12 Jul 2017
Date Written: November 17, 2014
Abstract
The study followed the implication of tidiazuron (TDZ) in the induction of embryogenic callus formation and plant regeneration from this callus. Three different concentrations of TDZ (0.5, 1.0 and 2.0 mg/l) were introduced in the basic Murashige-Skoog environment, each combined with an auxine (ANA), in a single 0.5 mg/l concentration. If on the witness sample (MS), the regeneration of a completely conformed plant occurs, on the variant with a 1 mg/l dose of TDZ, formation of a callus mass of circa 4 cm ø occurs, green, slightly firable, which after 40 days generates 2-3 small but completely conformed plantlets. In the higher concentration, of 2.0 mg/l TDZ, the nodal explant generated the same mass of olive green callus, friable, with a high number of embryoids on its surface. The fresh and dry weights, as well as the water content of the tissue highlighted the relationship between the callus water content and its friability. The uniformity of results regarding the obtainment of embryogenic callus and of plants from callus made possible the conlusion that TDZ has implications on the formation of embryogenic callus (V3) and on the regeneration of plants from callus (V2). Thus, tidiazuron in the culture medium, în moderate concentration of 1 mg/l, behaves like a cytochinine, with real implication on the regeneration of plants from callus.
Keywords: Dianthus spiculifolius Schur. tidiazuron (TDZ), naftil acetic acid, embryogenic callus, embryoids, regeneration, fresh weight, dry weight, water quantity
JEL Classification: A10
Suggested Citation: Suggested Citation