The Implications of Tidiazuron (TDZ) in the Induction of Callus and Plant Regeneration in the Dianthus Spiculifolius Schur. Variety

Analele Universităţii din Oradea, Fascicula Protecţia Mediului - Vol. XXI, 2013

8 Pages Posted: 12 Jul 2017

See all articles by Laslo Vasile

Laslo Vasile

University of Oradea

Zăpârţan Maria

University of Oradea

Eliza Maria Agud

Romanian Academy - Institute for World Economy

Simona Vicaş

University of Oradea

Date Written: November 17, 2014

Abstract

The study followed the implication of tidiazuron (TDZ) in the induction of embryogenic callus formation and plant regeneration from this callus. Three different concentrations of TDZ (0.5, 1.0 and 2.0 mg/l) were introduced in the basic Murashige-Skoog environment, each combined with an auxine (ANA), in a single 0.5 mg/l concentration. If on the witness sample (MS), the regeneration of a completely conformed plant occurs, on the variant with a 1 mg/l dose of TDZ, formation of a callus mass of circa 4 cm ø occurs, green, slightly firable, which after 40 days generates 2-3 small but completely conformed plantlets. In the higher concentration, of 2.0 mg/l TDZ, the nodal explant generated the same mass of olive green callus, friable, with a high number of embryoids on its surface. The fresh and dry weights, as well as the water content of the tissue highlighted the relationship between the callus water content and its friability. The uniformity of results regarding the obtainment of embryogenic callus and of plants from callus made possible the conlusion that TDZ has implications on the formation of embryogenic callus (V3) and on the regeneration of plants from callus (V2). Thus, tidiazuron in the culture medium, în moderate concentration of 1 mg/l, behaves like a cytochinine, with real implication on the regeneration of plants from callus.

Keywords: Dianthus spiculifolius Schur. tidiazuron (TDZ), naftil acetic acid, embryogenic callus, embryoids, regeneration, fresh weight, dry weight, water quantity

JEL Classification: A10

Suggested Citation

Vasile, Laslo and Maria, Zăpârţan and Agud, Eliza Maria and Vicaş, Simona, The Implications of Tidiazuron (TDZ) in the Induction of Callus and Plant Regeneration in the Dianthus Spiculifolius Schur. Variety (November 17, 2014). Analele Universităţii din Oradea, Fascicula Protecţia Mediului - Vol. XXI, 2013, Available at SSRN: https://ssrn.com/abstract=2692013

Laslo Vasile (Contact Author)

University of Oradea ( email )

Universitatii nr. 1
Oradea, Bihor 410087
Romania

Zăpârţan Maria

University of Oradea ( email )

Universitatii nr. 1
Oradea, Bihor 410087
Romania

Eliza Maria Agud

Romanian Academy - Institute for World Economy ( email )

Calea 13 Septembrie nr. 13
Sector 6
Bucharest, 050711
Romania

Simona Vicaş

University of Oradea ( email )

Universitatii nr. 1
Oradea, Bihor 410087
Romania

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