Gelatinases Expression Disturbance as a Possible Cause of Fibromuscular Dysplasia of Internal Carotid Arteries: Immunohistochemical Study

International Journal of Clinical and Experimental Medical Sciences, Vol. 2(4), pp. 52-58, 2016

7 Pages Posted: 22 Aug 2016

See all articles by Ekaterina M. Paltseva

Ekaterina M. Paltseva

The Russian National Research Center of Surgery

Viktoria O. Polyakova

D. O. Ott Research Institute of Obstetrics and Gynecology

Svetlana A. Oskolkova

I.M. Sechenov First Moscow State Medical University

Arsen V. Abramyan

The Russian National Research Center of Surgery

Julia S. Krylova

D. O. Ott Research Institute of Obstetrics and Gynecology

Alexandre V. Gavrilenko

The Russian National Research Center of Surgery

Igor M. Kvetnoy

D. O. Ott Research Institute of Obstetrics and Gynecology

Date Written: July 5, 2016

Abstract

Background: Fibromascular dysplasia of internal carotid arteries (ICA) leading to their pathological deformities is one of the causes of cerebral vascular insufficiency. The structural changes of the artery wall and their causes remain poorly understood.

Materials and Methods: We investigated the expression of elastin, collagen types I and III, smooth muscle cells, gelatinases degrading elastin (matrix metalloproteinases 2 and 9 (MMP2 and MMP9) and tissue inhibitors of matrix metalloproteinases 1 and 2 (TIMP1 and TIMP2) on formalin-fixed surgical samples with the methods of immunohistochemistry and confocal laser scanning microscopy.

Results: We revealed the fragmentation of elastic fibers (100% of patients) and some reduction of smooth muscle cells (p<0.05) in the tunica media of ICA. There were no changes in collagen types I and III and TIMP2 expression. The study of the ratio of the expression of MMPs and TIMPs revealed the statistically significant predominance of high MMP2 and -9 and low TIMP1 content in ICA with pathological deformities. With the use of confocal microscopy, we showed the decrease of elastin expression with a high MMP9 activity which correlated with low expression of TIMP-1 in the group of ICA with pathological deformities. While in the control group there was a high level of elastin expression and a low level of MMP9 expression that correlated with the low TIMP-1 amount (p<0.05).

Conclusion: Our data demonstrate that the main feature of fibromuscular dysplasia underlying the pathological deformities of ICA – fragmentation of elastic fibers – is caused by the disturbance of balance between gelatinases and their inhibitors.

Keywords: Pathological Deformities of Internal Carotid Artery, Elastin, Collagen, Smooth Muscle Cells, Matrix Metalloproteinase, Tissue Inhibitor of Matrix Metalloproteinases

Suggested Citation

Paltseva, Ekaterina M. and Polyakova, Viktoria O. and Oskolkova, Svetlana A. and Abramyan, Arsen V. and Krylova, Julia S. and Gavrilenko, Alexandre V. and Kvetnoy, Igor M., Gelatinases Expression Disturbance as a Possible Cause of Fibromuscular Dysplasia of Internal Carotid Arteries: Immunohistochemical Study (July 5, 2016). International Journal of Clinical and Experimental Medical Sciences, Vol. 2(4), pp. 52-58, 2016 , Available at SSRN: https://ssrn.com/abstract=2826216

Ekaterina M. Paltseva (Contact Author)

The Russian National Research Center of Surgery ( email )

Abrikosovskii per., 2
Moscow
Russia

Viktoria O. Polyakova

D. O. Ott Research Institute of Obstetrics and Gynecology ( email )

3 Mendeleyevskaya line
Saint Petersburg, 199034
Russia

Svetlana A. Oskolkova

I.M. Sechenov First Moscow State Medical University ( email )

Trubetskaya ul., 8
Moscow
Russia

Arsen V. Abramyan

The Russian National Research Center of Surgery ( email )

Abrikosovskii per., 2
Moscow
Russia

Julia S. Krylova

D. O. Ott Research Institute of Obstetrics and Gynecology ( email )

3 Mendeleyevskaya line
Saint Petersburg, 199034
Russia

Alexandre V. Gavrilenko

The Russian National Research Center of Surgery ( email )

Abrikosovskii per., 2
Moscow
Russia

Igor M. Kvetnoy

D. O. Ott Research Institute of Obstetrics and Gynecology ( email )

3 Mendeleyevskaya line
Saint Petersburg, 199034
Russia

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