Salt Tolerance of Genetic Modified Potato (Solanum Tuberosum) CV. Agria by Expression of a Bacterial MtLD Gene

Askari, A., Pepoyan, A., Parsaeimehr, A., 2012. Salt tolerance of genetic modified potato (Solanum tuberosum) cv. Agria by expression of a bacterial mtlD gene. Advances in Agriculture & Botanics 4(1):10-16.

7 Pages Posted: 16 Jun 2017

See all articles by Aliakbar Askari

Aliakbar Askari

Armenian State Agrarian University - Laboratory of Molecular Biology and Biotechnology

Astghik Pepoyan

Armenian State Agrarian University - Laboratory of Molecular Biology and Biotechnology

Ali Parsaeimehr

Delaware State University; Instituto Tecnológico de Monterrey

Date Written: March 13, 2012

Abstract

Water and salinity stresses are the major reasons to yield decreasing in the world. Potato is the world’s main tuber crops of the Solanaceae family which is one of the most economically important annual vegetable crop. The goal of this investigation was creation genetic modified potato cv. Agria with more tolerance to salinity stress and evaluating GMO potato properties. To create transgenic potato plant, mtlD gene (mannitol-1-phosphate dehydrogenase, E.C.1.1.1.17) was expressed to potato cv. Agria plant by using Agrobacterium tumefaciens. Transgenic potato was produced by transforming of mtlD gene to potato plant cv. Agria. Existence of recombinant gene in transgenic plants was approved by two ways 1. Polymerase Chain Reaction technique. 2. Measurement of physiological parameters. The transgenic potatoes and non-transgenic potatoes lines exhibited the different amounts of tolerance to salinity stress because in the transgenic lines mannitol accumulates that increased osmotic pressure in salinity stress. The salt tolerance of transgenic potato cv. Agria ( mtlD) was recorded higher than that of non-transgenic potato cv. Agria (-mtlD). Osmotic pressure in this transgenic potato plant was increased by accumulating of mannitol and existence of mannitol in potato plant approved that the mtlD gene was successfully expressed to potato cv. Agria.

Keywords: transgenic potato, mtlD gene, salinity stress, callus culture

Suggested Citation

Askari, Aliakbar and Pepoyan, Astghik and Parsaeimehr, Ali, Salt Tolerance of Genetic Modified Potato (Solanum Tuberosum) CV. Agria by Expression of a Bacterial MtLD Gene (March 13, 2012). Askari, A., Pepoyan, A., Parsaeimehr, A., 2012. Salt tolerance of genetic modified potato (Solanum tuberosum) cv. Agria by expression of a bacterial mtlD gene. Advances in Agriculture & Botanics 4(1):10-16., Available at SSRN: https://ssrn.com/abstract=2987429

Aliakbar Askari

Armenian State Agrarian University - Laboratory of Molecular Biology and Biotechnology ( email )

Armenia

Astghik Pepoyan

Armenian State Agrarian University - Laboratory of Molecular Biology and Biotechnology

Armenia

Ali Parsaeimehr (Contact Author)

Delaware State University ( email )

Dover, DE 19901
United States

Instituto Tecnológico de Monterrey ( email )

School of Engineering and Sciences, Tecnologico de
Monterrey, Nuevo León
Mexico

HOME PAGE: http://https://mx.linkedin.com/in/ali-parsaeimehr-822b173a

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