Massachusetts Institute of Technology (MIT) - Howard Hughes Medical Institute (HHMI); Massachusetts Institute of Technology (MIT) - Department of Biology
Helmholtz Centre for Infection Research - Nanoscale Infection Biology; École Polytechnique Fédérale de Lausanne - Institute of Physics; National Centre for Competence in Research Programme Chemical Biology
Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls, then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed constriction rate using a hyperconstriction mutant or fosfomycin inhibition. We report that constriction rate contributes to both size control and homeostasis, by determining elongation during constriction, and by compensating for variation in pre-constriction elongation on a single-cell basis.
Lambert, Ambroise and Vanhecke, Aster and Archetti, Anna and Holden, Seamus and Schaber, Felix and Pincus, Zachary and Laub, Michael T. and Laub, Michael T. and Goley, Erin and Manley, Suliana, Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus (2018). Available at SSRN: https://ssrn.com/abstract=3155796 or http://dx.doi.org/10.2139/ssrn.3155796
This version of the paper has not been formally peer reviewed.
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