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A Genetic, Genomic, and Computational Resource for Exploring Neural Circuit Function

92 Pages Posted: 17 Aug 2018 Publication Status: Review Complete

See all articles by Fred P. Davis

Fred P. Davis

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Aljoscha Nern

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Serge Picard

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Michael B. Reiser

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Gerald M. Rubin

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Sean R. Eddy

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

Gilbert L. Henry

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

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Abstract

The anatomy of many neural circuits is being characterized with increasing resolution, but their molecular properties remain mostly unknown. Here, we characterize gene expression patterns in distinct neural cell types of the Drosophila visual system using genetic lines to access individual cell types, the TAPIN-seq method to measure their transcriptomes, and a probabilistic method to interpret these measurements. We used these tools to build a resource of high-resolution transcriptomes for 100 driver lines covering 67 cell types. Combining these transcriptomes with recently reported connectomes helps characterize how information is transmitted and processed across a range of scales, from individual synapses to circuit pathways. We describe examples that include identifying neurotransmitters, including cases of co-release, generating functional hypotheses based on receptor expression, as well as identifying strong commonalities between different cell types.

Suggested Citation

Davis, Fred P. and Nern, Aljoscha and Picard, Serge and Reiser, Michael B. and Rubin, Gerald M. and Eddy, Sean R. and Henry, Gilbert L., A Genetic, Genomic, and Computational Resource for Exploring Neural Circuit Function (2018). Available at SSRN: https://ssrn.com/abstract=3232155 or http://dx.doi.org/10.2139/ssrn.3232155
This version of the paper has not been formally peer reviewed.

Fred P. Davis (Contact Author)

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus ( email )

19700 Helix Drive
Ashburn, VA 20147
United States

Aljoscha Nern

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

19700 Helix Drive
Ashburn, VA 20147
United States

Serge Picard

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

19700 Helix Drive
Ashburn, VA 20147
United States

Michael B. Reiser

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

19700 Helix Drive
Ashburn, VA 20147
United States

Gerald M. Rubin

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

19700 Helix Drive
Ashburn, VA 20147
United States

Sean R. Eddy

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus

19700 Helix Drive
Ashburn, VA 20147
United States

Gilbert L. Henry

Howard Hughes Medical Institute (HHMI) - Chevy Chase - Janelia Research Campus ( email )

19700 Helix Drive
Ashburn, VA 20147
United States

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