puc-header

Wide Cross-Species RNA-Seq Comparison Reveals Convergent Molecular Mechanisms Involved in Nickel Hyperaccumulation Across Angiosperms

34 Pages Posted: 24 Oct 2018 Publication Status: Under Review

See all articles by Vanesa S. Garcia de la Torre

Vanesa S. Garcia de la Torre

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

Clarisse Majorel-Loulergue

Université de la Nouvelle-Calédonie - Institute of Exact and Applied Sciences (ISEA)

Dubiel A. Gonzalez

Universidad Agraria de La Habana (UNAH) - Departamento de Biologia

Ludivine Soubigou-Taconnat

University of Paris-Saclay - Institute of Plant Sciences Paris-Saclay (IPS2)

Guillem J. Rigaill

University of Paris-Saclay - Institute of Plant Sciences Paris-Saclay (IPS2)

Yohan Pillon

University of Montpellier - Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM)

Louise Barreau

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

Sébastien Thomine

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

Bruno Fogliani

Equipe ARBOREAL - Institut Agronomique néo-Calédonien (IAC)

Valérie Burtet-Sarramegna

Université de la Nouvelle-Calédonie - Institute of Exact and Applied Sciences (ISEA)

Sylvain Merlot

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

More...

Abstract

The Anthropocene epoch is associated with the spreading of metals in the environment increasing oxidative and genotoxic stress on living organisms. Metalliferous soils such as serpentine can host peculiar plants called hyperaccumulators due to their capacity to accumulate tremendous amount of metal in their shoot (e.g. >0.1% of dry weight for nickel). Once regarded as a curiosity, plants hyperaccumulating metals are now envisioned as an opportunity to remediate metal contaminated soils. The wide phylogenetic distribution of nickel hyperaccumulators (~500 species) suggest that this complex trait evolved multiple times independently from the dysregulation of basic mechanisms involved in metal homeostasis including metal chelation, transport and oxidative stress responses. However, the exact nature of these mechanisms and whether the same molecular mechanisms have been recruited convergently to reach this extreme trait is not known. To address these questions, we developed a comparative cross-species RNA-Seq approach combining differential gene expression analysis and cluster of orthologous group annotation. This analysis reveals candidate orthologous genes encoding convergent function, including the biosynthesis of histidine and flavonoids, involved in nickel hyperaccumulation. Our data also point out that the constitutive high expression of IREG/Ferroportin transporters recurrently emerged as a mechanism involved in nickel hyperaccumulation in a wide diversity of plant families. We further provide genetic evidence in the hyperaccumulator Noccaea caerulescens that IREG/Ferroportin transporters are involved in nickel sequestration in vacuoles. This study paves the way for comparative approaches to identify molecular mechanisms involved in metal hyperaccumulation in a wide diversity of plant species.

Keywords: Hyperaccumulator, Metal, Nickel, RNA-Seq, Cluster of Orthologous Groups, IREG/Ferroportin, Flavonoids, Convergence, Noccaea caerulescens

Suggested Citation

de la Torre, Vanesa S. Garcia and Majorel-Loulergue, Clarisse and Gonzalez, Dubiel A. and Soubigou-Taconnat, Ludivine and Rigaill, Guillem J. and Pillon, Yohan and Barreau, Louise and Thomine, Sébastien and Fogliani, Bruno and Burtet-Sarramegna, Valérie and Merlot, Sylvain, Wide Cross-Species RNA-Seq Comparison Reveals Convergent Molecular Mechanisms Involved in Nickel Hyperaccumulation Across Angiosperms (October 24, 2018). Available at SSRN: https://ssrn.com/abstract=3272237 or http://dx.doi.org/10.2139/ssrn.3272237
This version of the paper has not been formally peer reviewed.

Vanesa S. Garcia De la Torre

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

55 Avenue de Paris
Versailles, 78000
France

Clarisse Majorel-Loulergue

Université de la Nouvelle-Calédonie - Institute of Exact and Applied Sciences (ISEA)

BPR4 - 98851 Nouméa Cedex
New Caledonia

Dubiel A. Gonzalez

Universidad Agraria de La Habana (UNAH) - Departamento de Biologia

San Lazaro
Havana
Cuba

Ludivine Soubigou-Taconnat

University of Paris-Saclay - Institute of Plant Sciences Paris-Saclay (IPS2)

Bâtiment 630
Plateau de Moulon
Gif sur Yvette, 91192
France

Guillem J. Rigaill

University of Paris-Saclay - Institute of Plant Sciences Paris-Saclay (IPS2)

Bâtiment 630
Plateau de Moulon
Gif sur Yvette, 91192
France

Yohan Pillon

University of Montpellier - Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM)

163 rue Auguste Broussonnet
France

Louise Barreau

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

55 Avenue de Paris
Versailles, 78000
France

Sébastien Thomine

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC)

55 Avenue de Paris
Versailles, 78000
France

Bruno Fogliani

Equipe ARBOREAL - Institut Agronomique néo-Calédonien (IAC)

BP 73
Païta, 98890
New Caledonia

Valérie Burtet-Sarramegna

Université de la Nouvelle-Calédonie - Institute of Exact and Applied Sciences (ISEA)

BPR4 - 98851 Nouméa Cedex
New Caledonia

Sylvain Merlot (Contact Author)

University of Paris-Saclay - Institute for Integrative Biology of the Cell (I2BC) ( email )

55 Avenue de Paris
Versailles, 78000
France

Click here to go to Cell.com

Paper statistics

Downloads
30
Abstract Views
680
PlumX Metrics