Mechanical Characterization of an In-Body Tissue-Engineered Autologous Collagenous Sheet, Biosheet, for Application as an Aortic Valve Reconstruction Material

22 Pages Posted: 24 Jan 2019

See all articles by Takeshi Terazawa

Takeshi Terazawa

Hokkaido University - Division of Cell Engineering; National Cerebral and Cardiovascular Centre - Department of Regenerative Medicine and Tissue Engineering; Biotube Co., Ltd.

Takayuki Kawashima

Oita University - Department of Cardiovascular Surgery

Tadashi Umeno

Oita University - Department of Cardiovascular Surgery

Tomoyuki Wada

Oita University - Department of Cardiovascular Surgery

Shigeyuki Ozaki

Toho University - Department of Cardiovascular Surgery

Shinji Miyamoto

Oita University - Department of Cardiovascular Surgery

Yasuhide Nakayama

Hokkaido University - Division of Cell Engineering; Biotube Co., Ltd.; Oita University - Department of Cardiovascular Surgery

Date Written: January 22, 2019

Abstract

The reconstruction of the aortic valve using glutaraldehyde-treated autologous pericardium is known as “aortic valve neo-cuspidization” (AVNeo). In-body tissue architecture (iBTA), a cell-free, in vivo tissue-engineering that can produce autologous implantable tissues of the desired shape by subcutaneously embedding specially designed molds, was used to develop sheet-like collagenous tissues called “Biosheets.” Cylindrical molds with line slits arranged in an alternating (n=30) or parallel (n=36) pattern were subcutaneously embedded in goats (n=12) for several months. The tubular tissues formed in the molds were dried and then cut in the longitudinal direction, thus forming the sheet-like Biosheets (5×7 cm). The success rate was 97.6% when using the alternating-pattern molds and 97.2% for the parallel molds. Thickness mapping of the Biosheets showed that their entire surface, except for the line-projection portions, was smooth without any defects. The average wall thickness could be controlled over a range of ca. 0.2–0.5 mm by changing the size of the gap (0.75–1.5 mm) in the molds. The alternating slit-patterned Biosheets were found to be almost isotropic in their mechanical properties (ultimate tensile strength, fracture strain, and Young’s modulus). Although the composition of the Biosheet wall was heterogeneous in terms of its density, the breaking force of all the alternating-patterned Biosheets increased almost linearly with the thickness within the range of the thickness of glutaraldehyde-treated human pericardium, and was larger than that of human aortic valve leaflets. Therefore, the alternating-patterned Biosheets may be used as an alternative aortic leaflet material in AVNeo.

Keywords: aortic valve reconstruction, AVNeo, in-body tissue architecture, biosheet, tissue engineering

Suggested Citation

Terazawa, Takeshi and Kawashima, Takayuki and Umeno, Tadashi and Wada, Tomoyuki and Ozaki, Shigeyuki and Miyamoto, Shinji and Nakayama, Yasuhide, Mechanical Characterization of an In-Body Tissue-Engineered Autologous Collagenous Sheet, Biosheet, for Application as an Aortic Valve Reconstruction Material (January 22, 2019). Available at SSRN: https://ssrn.com/abstract=3320740 or http://dx.doi.org/10.2139/ssrn.3320740

Takeshi Terazawa

Hokkaido University - Division of Cell Engineering

5 Kita 8 Jonishi, Kita Ward
Hokkaido Prefecture
Sapporo, Hokkaido 060-0808
Japan

National Cerebral and Cardiovascular Centre - Department of Regenerative Medicine and Tissue Engineering

5-7-1 Fujishiro-dai, Suita
Osaka, 565-8565
Japan

Biotube Co., Ltd.

Romford, Essex
United Kingdom

Takayuki Kawashima

Oita University - Department of Cardiovascular Surgery

700 Dannoharu
Oita, 870-1124
Japan

Tadashi Umeno

Oita University - Department of Cardiovascular Surgery

700 Dannoharu
Oita, 870-1124
Japan

Tomoyuki Wada

Oita University - Department of Cardiovascular Surgery

700 Dannoharu
Oita, 870-1124
Japan

Shigeyuki Ozaki

Toho University - Department of Cardiovascular Surgery

5-21-16 Omorinishi
Tokyo, Ōta 143-8540
Japan

Shinji Miyamoto

Oita University - Department of Cardiovascular Surgery

700 Dannoharu
Oita, 870-1124
Japan

Yasuhide Nakayama (Contact Author)

Hokkaido University - Division of Cell Engineering ( email )

5 Kita 8 Jonishi, Kita Ward
Hokkaido Prefecture
Sapporo, Hokkaido 060-0808
Japan

Biotube Co., Ltd. ( email )

Romford, Essex
United Kingdom

Oita University - Department of Cardiovascular Surgery ( email )

700 Dannoharu
Oita, 870-1124
Japan

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