Hepatoprotective Effect of DL-Methionine on Diclofenac-Induced Hepatotoxicity in Albino Rats: An Experimental Study

Posted: 1 May 2019

See all articles by Bhagya Sattigeri

Bhagya Sattigeri

Dr KC Patel Medical College & Research Institute

Date Written: March 31, 2019

Abstract

Background: Liver is the main detoxifying organ, which is affected by most of the drugs and xenobiotic agents that could result in liver damage. The present study was designed to evaluate the hepatoprotective effect of DL-Methionine against experimentally induced liver injury in albino rats.

Methods: Hepatotoxicity was induced by administering high doses of positive control drug Diclofenacsodium in albino rats, which was confirmed by estimating Liver Function Tests. Hepato protective effect was determined by administering DL-Methionine concomitantly with positive control drug. Albino rats were administered with DL-Methionine (700mg/kgand1400mg/kg) respectively as a single oral dose, concomitantly with positive control drug Diclofenacsodium (96mg/kgand240mg/kg) respectively. After 24-hours of post-treatment, serum levels of the liver enzymes were evaluated to demonstrate the hepato protective effect of DL-Methionineon drug-induced hepatotoxicity, and all the liver samples were examined for the histopathological study.

Results: Significant increase in serum transaminase enzymes were observed by the positive control drug Diclofenacsodium. There was significant reduction in the serum transaminaseson concomitant administration of DL-Methionine with Diclofenacsodium. Liver injury induced by positive control drug; and it sprotection with DL-Methionine was revealed by histopathological study. The combination of Diclofenacsodium and DL-Methionine showed no significant histopathological difference when compared to the normal liver section.

Conclusions: The results reveal that,DL-Methionine significantly prevented the rise in transaminases levels produced by hepatotoxic doses of the positive control drug.

Keywords: Diclofenac, Drug-Induced Hepatotoxicity, Liver Injury, NSAIDs, Serum Markers

Suggested Citation

Sattigeri, Bhagya, Hepatoprotective Effect of DL-Methionine on Diclofenac-Induced Hepatotoxicity in Albino Rats: An Experimental Study (March 31, 2019). Available at SSRN: https://ssrn.com/abstract=3363154

Bhagya Sattigeri (Contact Author)

Dr KC Patel Medical College & Research Institute ( email )

B-101, B-Wing, Nishad -2, Ellora Park, Vadodara
Bharuch
India
9426234943 (Phone)

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