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Clinical-Grade Garcinia cambogia Extract Removes Calcium Oxalate Kidney Stones in Both Genetic and Non-Genetic Drosophila Models of Nephrolithiasis

30 Pages Posted: 17 May 2019

See all articles by Qiuxia Fan

Qiuxia Fan

Southern Medical University - State Key Laboratory of Organ Failure Research

Xiaoming Feng

Southern Medical University - State Key Laboratory of Organ Failure Research

Xizhen Hong

Southern Medical University - State Key Laboratory of Organ Failure Research

Siqiao Gong

Southern Medical University - State Key Laboratory of Organ Failure Research

Jianwei Tian

Southern Medical University - State Key Laboratory of Organ Failure Research

Fan Fan Hou

Southern Medical University, Nanfang Hospital, Division of Nephrology, State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease

Fujian Zhang

Southern Medical University - State Key Laboratory of Organ Failure Research; Southern Medical University - National Clinical Research Center of Kidney Diseases

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Abstract

BackgroundKidney stone formers with family history have a high rate of stone recurrence after kidney stone removal surgery and there is no effective medication available for treatment. It has been shown that hydroxycitric acid (HCA)-the major component of Garcinia cambogia extract, induces dissolution of the calcium oxalate crystal in vitro, suggesting that clinical-grade Garcinia cambogia has the potential to treat calcium oxalate kidney stone. In this study, we used the Drosophila genetic and non-genetic nephrolithiasis model to evaluate the effect of Garcinia cambogia and HCA on the prevention and removal of calcium oxalate stones in vivo.

Methods: WT flies were reared in fly food with different concentrations of GCE for one week.The effect of GCE on preventing the formation of calcium oxalate stone was examined. WT and v-ATPase gene knockdown flies were reared in fly food containing 0.3% NaOx for one week, then fed different concentrations of GCE for one week.The effect of GCE on the removal of calcium oxalate stone was examined.

Findings: Garcinia cambogia extract efficiently removes calcium oxalate kidney stones from Malpighian tubules in both genetic and non-genetic Drosophila models of nephrolithiasis in vivo. HCA directly dissolves calcium oxalate stones in Drosophila Malpighian tubules ex vivo.

Interpretation: Clinical-grade Garcinia cambogia extract removes calcium oxalate kidney stones from Drosophila Malpighian tubules via directly dissolving calcium oxalate stones by HCA. Our study strongly suggests that clinical-grade Garcinia cambogia extract could be used to treat patients with nephrolithiasis in the future.

Funding Statement: F.J.Z. was supported by grants from National Key Research Plan (2017YFA0104602) and F.F.H was supported by the National Key Technology Support Program of China (2013BAI09B06 and 2015BAI2B07), the State Key Program of National Natural Science Foundation of China (81430016), the Major International (Regional) Joint Research Project of National Natural Science Foundation of China (81620108003), the Major Scientific and Technological Planning Project of Guangzhou (15020010), and the Guangzhou Clinical Research Center for Chronic Kidney Disease Program (7415695988305).

Declaration of Interests: The authors declare that they have no competing interests.

Ethics Approval Statement: The authors state: "Not applicable"

Keywords: Garcinia cambogia extract; Hydroxycitrate; Calcium oxalate kidney stone; Nephrolithiasis; Drosophila genetic model; Malpighian tubule

Suggested Citation

Fan, Qiuxia and Feng, Xiaoming and Hong, Xizhen and Gong, Siqiao and Tian, Jianwei and Hou, Fan Fan and Zhang, Fujian, Clinical-Grade Garcinia cambogia Extract Removes Calcium Oxalate Kidney Stones in Both Genetic and Non-Genetic Drosophila Models of Nephrolithiasis (May 14, 2019). Available at SSRN: https://ssrn.com/abstract=3388806 or http://dx.doi.org/10.2139/ssrn.3388806

Qiuxia Fan

Southern Medical University - State Key Laboratory of Organ Failure Research

Guangzhou, Guangdong Province
China

Xiaoming Feng

Southern Medical University - State Key Laboratory of Organ Failure Research

Guangzhou, Guangdong Province
China

Xizhen Hong

Southern Medical University - State Key Laboratory of Organ Failure Research

Guangzhou, Guangdong Province
China

Siqiao Gong

Southern Medical University - State Key Laboratory of Organ Failure Research

Guangzhou, Guangdong Province
China

Jianwei Tian

Southern Medical University - State Key Laboratory of Organ Failure Research

Guangzhou, Guangdong Province
China

Fan Fan Hou

Southern Medical University, Nanfang Hospital, Division of Nephrology, State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease ( email )

Guangzhou, Guangdong Province
China

Fujian Zhang (Contact Author)

Southern Medical University - State Key Laboratory of Organ Failure Research ( email )

Guangzhou, Guangdong Province
China

Southern Medical University - National Clinical Research Center of Kidney Diseases ( email )

China

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