Isolation, Characterization and Partial Purification of Keratinase from Keratinolytic Bacteria

Scholar Journal of Applied Sciences and Research, 2018, Vol. 1:6

6 Pages Posted: 4 Jun 2019

See all articles by Prakash Bhuyar

Prakash Bhuyar

Maejo University; Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia; Garden City College (India), Students

Sourabh Zagde

Garden City College (India)

Rajesh Revankar

Garden City College (India)

Mashitah Yusoff

University Malaysia Pahang - Faculty of Industrial Sciences & Technology

Mohd Hasbi Ab. Rahim

Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia

Natanamurugaraj Govindan

Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia

Date Written: September 11, 2018

Abstract

Background: Enzymes are proteins that catalyze the chemical reactions, such as biosynthetic and derivative, that occurring in living cells. In enzymatic reactions, the molecules at the beginning of process are called substrates and they are converted in to different molecules, called the product. Almost all processes in a biological cell need enzyme for catalytic reactions. The use of purified enzymes for generating a useful product or service constitutes enzyme technology. Enzymes have a wide variety of applications in industry, medicine research etc. Some out of this one industrially important enzyme is keratinase. It is an extracellular and proteolytic enzyme.

Methods: Keratinolytic bacteria were isolated from poultry contaminated soil identification done by using morphological methods and biochemical test. Enzyme production was done followed by partial purification methods like salting out technique, dialysis and Ion exchange chromatography.

Results: The bacterial strains were isolated from the poultry waste and evaluated for their Keratinolytic activity on skim milk agar media. The keratin degrading bacteria or keratinolytic bacterial culture was isolated from soil sample and Colony characteristics, Gram nature and motility of the keratinolytic culture were studied. It is Gram negative short rods, non-motile in nature and colony characteristics and morphology was studied. The biochemical testes of keratinolytic culture were also studied and their results were found. The enzyme production was observed between temperature 25-50°C and pH 5.5-9.5 The highest enzyme activity was observed at 35°C, decrease in enzyme activity is observed with increase in temperature above 40 0C. The highest enzyme activity was obtained at pH 7. However, further increase in pH was not favorable on enzyme production. It was reported that proteases secreted by Genus Pseudomonas presented activity at a wide range of pH (7.0 to 9.0) and temperature (30°C to 40°C).

Keywords: Azokeratin, Keratin, Keratinolytic bacteria.

Suggested Citation

Bhuyar, Prakash and Bhuyar, Prakash and Zagde, Sourabh and Revankar, Rajesh and Yusoff, Mashitah and Ab. Rahim, Mohd Hasbi and Govindan, Natanamurugaraj, Isolation, Characterization and Partial Purification of Keratinase from Keratinolytic Bacteria (September 11, 2018). Scholar Journal of Applied Sciences and Research, 2018, Vol. 1:6, Available at SSRN: https://ssrn.com/abstract=3389068 or http://dx.doi.org/10.2139/ssrn.3389068

Prakash Bhuyar (Contact Author)

Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia ( email )

Lebuhraya Tun Razak
Kuantan, Pahang 26600
Malaysia

Maejo University ( email )

Nong Han
San Sai
Chiang Mai, 50290
Thailand

HOME PAGE: http://www.prakashbhuyar.com

Garden City College (India), Students ( email )

India

Sourabh Zagde

Garden City College (India) ( email )

Bengaluru
Karnataka
India

Rajesh Revankar

Garden City College (India) ( email )

Bengaluru
Karnataka
India

Mashitah Yusoff

University Malaysia Pahang - Faculty of Industrial Sciences & Technology ( email )

Lebuhraya Tun Razak
Gambang
Kuantan, Pahang Darul 26300
Malaysia

Mohd Hasbi Ab. Rahim

Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia ( email )

Lebuhraya Tun Razak
Kuantan, Pahang 26600
Malaysia

Natanamurugaraj Govindan

Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia ( email )

Lebuhraya Tun Razak
Kuantan, Pahang 26600
Malaysia

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