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Early-Life Supplementation of Grape Pomace Extracts Lastingly Promotes Polyphenol Metabolism and Optimizes Gut Microbiota

55 Pages Posted: 18 Jun 2019

See all articles by Feng Lu

Feng Lu

China Agricultural University - College of Food Science and Nutritional Engineering

Yuanyuan Li

China Agricultural University - College of Food Science and Nutritional Engineering

Bing Zhou

Hebrew University of Jerusalem - Institute for Medical Research Israel-Canada

Qing Guo

China Agricultural University - College of Food Science and Nutritional Engineering

Fang Chen

China Agricultural University - College of Food Science and Nutritional Engineering

Xiaosong Hu

China Agricultural University - Beijing Advanced Innovation Center for Food Nutrition and Human Health

Yan Zhang

China Agricultural University - College of Food Science and Nutritional Engineering; National Engineering Research Center for Fruits and Vegetables Processing; Key Laboratory of Fruits and Vegetables Processing

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Abstract

Background: Previous investigations have reported that nutritional supplementation in a critical developmental window can strikingly influence health level and metabolic status in adult life. Grape polyphenol harbors many physiological properties, especially in regulating metabolic status. In the present study, we aimed to investigate whether the GPE supplementation in a vital window plays an important role in metabolic and health status in adult life, by promoting the polyphenol metabolism and optimizing host gut microbiota.

Methods: One group of 3-week-old mice and one group of 6-week-old mice were given GPE by gavage for 12 days, and the other group of 3-week- or 6-week- old mice were given by normal saline as control. We detected polyphenol metabolites in the plasma by UPLC-MS/MS. We also used Real-time quantitative RT-PCR to examine mRNA expression levels of genes related to polyphenol metabolism. Furthermore, we analysed the structure of gut microbiota and the correlation between the mRNA expression levels of polyphenol metabolism related genes and gut microbiota.

Findings: There were a considerably more abundance of polyphenol metabolites in plasma of 3-week-old mice than that in plasma of 6-week-old mice. Consequently, much higher mRNA expression levels of polyphenol metabolism related genes were observed in intestine of 3-week-old mice. Moreover, after GPE supplementation, the gut microbiota of 3-week-old mice was characterized by a drastic expansion of Akkermansia and Lactobacillus, and a remarkable inhibition of norank_f__Lachnospiraceae, unclassified_f__Lachnospiraceae, Mucispirillum, in comparison with that of 6-week-old mice. In addition, Akkermansia and Lactobacillus had the positive connections with the mRNA expression levels of genes related to polyphenol metabolism in jejunum, such as LPH, SGLT1, SULT1A1, UGT1A1, UGT1A4, UGT1A6.

Interpretation: GPE supplementation in a critical developmental window promoted polyphenol absorption and modulated gut microbial community structure, which might contribute to metabolic status and the long-term health.

Funding Statement: National Key Research and Development Plan during the 13th five-year plan period (No. 2017YFD0400504).

Declaration of Interests: The authors declare no conflict of interest.

Ethics Approval Statement: The animal study was approved by the China Agricultural University Laboratory Animal Welfare and Animal Experimental Ethical Committee (Beijing, China).

Keywords: Grape pomace extracts; Metabolic status; Critical developmental window; Polyphenol metabolism; Gut microbiota

Suggested Citation

Lu, Feng and Li, Yuanyuan and Zhou, Bing and Guo, Qing and Chen, Fang and Hu, Xiaosong and Zhang, Yan, Early-Life Supplementation of Grape Pomace Extracts Lastingly Promotes Polyphenol Metabolism and Optimizes Gut Microbiota (June 17, 2019). Available at SSRN: https://ssrn.com/abstract=3405555 or http://dx.doi.org/10.2139/ssrn.3405555

Feng Lu

China Agricultural University - College of Food Science and Nutritional Engineering

Beijing
China

Yuanyuan Li

China Agricultural University - College of Food Science and Nutritional Engineering

Beijing
China

Bing Zhou

Hebrew University of Jerusalem - Institute for Medical Research Israel-Canada

Jerusalem
Israel

Qing Guo

China Agricultural University - College of Food Science and Nutritional Engineering

Beijing
China

Fang Chen

China Agricultural University - College of Food Science and Nutritional Engineering

Beijing
China

Xiaosong Hu

China Agricultural University - Beijing Advanced Innovation Center for Food Nutrition and Human Health

Beijing
China

Yan Zhang (Contact Author)

China Agricultural University - College of Food Science and Nutritional Engineering ( email )

Beijing
China

National Engineering Research Center for Fruits and Vegetables Processing ( email )

China

Key Laboratory of Fruits and Vegetables Processing ( email )

China