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Exploring Lens-Injury-Derived Effect on Optic Nerve Regeneration after Optic Nerve Lesion with Cataractogenic Lens Injury by the RNA-Seq Profiling

25 Pages Posted: 19 Jul 2019

See all articles by Kang Liu

Kang Liu

920th Hospital of Joint Logistics Support Force

Yu Geng

Kunming Medical University - Ophthalmology Department

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Abstract

Background: Regeneration after optic nerve injury is a quite difficult problem in regenerative medicine for a long time. While the optic nerves had been damaged accompanied with the lens injury, it can start the long-distance axonal growth pattern. However, the lens-injury-derived effect on optic nerve injury regeneration have not been revealed yet.

Methods: The SD rats were used to form optic nerve injury and/or lens injury. After two weeks later, orbital optic nerves of these rats were taken out carefully from the optic sheath, which contain the area of optic nerve injury, approximate 1-2 mm length. The total RNA of optic nerve tissues was extracted. After RNA qualification and library preparation for transcriptome sequencing, Quantification of gene expression level, and differential gene expression analysis, and GO and KEGG enrichment analysis were be executed.

Findings: The results of differential expression analysis, GO and KEGG enrichment analysis mainly exhibited that after lens injury, the expression patterns of optic nerve injury tissues had changed, and the local cellular inflammatory system, including neutrophil and macrophage, were activated, which manifested the upregulated expression of MMP-8 and MMP-12 genes.

Interpretation: We speculated that the release of crystalline proteins interacted with infiltrating macrophages and other inflammatory cells, then the inflammatory cells enter the blood circulation, and reach the site of optic nerve injury, finally release MMP-8 and MMP-12, which play the role of not only lytic extracellular matrix, but also axon guidance. The ultimate effect is to promote the regeneration of the optic nerve.

Funding Statement: This study was supported by the National Natural Science Foundation of China (grant no. 81560160) grant funded by China government.

Declaration of Interests: Dr. Kang Liu has nothing to disclose; Dr. Yu Geng has nothing to disclose.

Ethics Approval Statement: The care and maintenance of the animals conformed to the Association for Research in Vision and Ophthalmology Statement, to the Animal Welfare, and to the Animal Ethics Committee of Kunming Medical University.

Keywords: Optic nerve regeneration; Cataractogenic lens injury; RNA-Seq; Matrix metalloproteinase-8; Matrix metalloproteinase-12

Suggested Citation

Liu, Kang and Geng, Yu, Exploring Lens-Injury-Derived Effect on Optic Nerve Regeneration after Optic Nerve Lesion with Cataractogenic Lens Injury by the RNA-Seq Profiling (07/16/2019 13:35:20). Available at SSRN: https://ssrn.com/abstract=3421584 or http://dx.doi.org/10.2139/ssrn.3421584

Kang Liu

920th Hospital of Joint Logistics Support Force

China

Yu Geng (Contact Author)

Kunming Medical University - Ophthalmology Department ( email )

China

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