Antibacterial Activity and Sensory Properties of Heracleum Persicum Essential Oil, Nisin and Lactobacillus Acidophilus Against Listeria Monocytogenes in Cheese

Veterinary World, 2019

7 Pages Posted: 5 Aug 2019

See all articles by Ali Ehsani

Ali Ehsani

Tabriz University of Medical Sciences

Ahmad Rezaeiyan

Urmia University

Mohammad Hashemi

Mashhad University of Medical Sciences

Majid Aminzare

Zanjan University of Medical Sciences

Behrooz Jannat

Halal Research Center of IRI

Asma Afshari

Mashhad University of Medical Sciences

Date Written: July 19, 2019

Abstract

Aim: The aim of this study was to evaluate the antibacterial and chemical effect of Heracleum persicum essential oil (EO), nisin, Lactobacillus acidophilus, and their combination against Listeria monocytogenes both in vitro and in Iranian white cheese model.

Materials and Methods: Chemical compositions of H. persicum EO were analyzed by gas chromatography–mass spectrometry. After production of Iranian white cheese, minimum inhibitory concentration (MIC) and minimum bactericidal concentration of EO and nisin and agar spot test of L. acidophilus against L. monocytogenes were evaluated.

Results: Hexyl butanoate (25.98%), octyl isobutyrate (17.82%), methyl butyrate (14.37%), and pentyl cyclopropane (12.77%) were the main components of the EO. MIC of the EO against L. monocytogenes was 2.5 mg/mL. Combination of nisin (5.3 IU/mL) and H. persicum EO (2500 μg/mL) showed increasing effect against L. monocytogenes (fractional inhibitory concentration = 0.9), while a higher concentration of EO and nisin showed undesirable effect on the cheese flavor. Furthermore, a combination of 1012 CFU/g L. acidophilus with H. persicum EO at the concentration of 2.5 mg/mL (T12) showed acceptable sensorial and also antibacterial results in Iranian white cheese.

Keywords: Heracleum persicum, Lactobacillus acidophilus, Listeria monocytogenes, Nisin

Suggested Citation

Ehsani, Ali and Rezaeiyan, Ahmad and Hashemi, Mohammad and Aminzare, Majid and Jannat, Behrooz and Afshari, Asma, Antibacterial Activity and Sensory Properties of Heracleum Persicum Essential Oil, Nisin and Lactobacillus Acidophilus Against Listeria Monocytogenes in Cheese (July 19, 2019). Veterinary World, 2019, Available at SSRN: https://ssrn.com/abstract=3422783

Ali Ehsani

Tabriz University of Medical Sciences ( email )

Tabriz
Iran

Ahmad Rezaeiyan

Urmia University ( email )

daneshkade street
Urmia, West Azarbaijan
Iran

Mohammad Hashemi

Mashhad University of Medical Sciences ( email )

Mashhad
Iran

Majid Aminzare (Contact Author)

Zanjan University of Medical Sciences ( email )

Iran

Behrooz Jannat

Halal Research Center of IRI ( email )

Iran

Asma Afshari

Mashhad University of Medical Sciences ( email )

Mashhad
Iran

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