header

Design of Injectable Hydrogels of Gelatin and Alginate with Ferric Ions for Cell Transplantation

33 Pages Posted: 24 Jul 2019 Publication Status: Accepted

See all articles by Mina Anamizu

Mina Anamizu

Kyoto University - Laboratory of Biomaterials

Yasuhiko Tabata

Kyoto University - Laboratory of Biomaterials

Abstract

The objective of this study is to design bioabsorbable injectable hydrogels based on the physico-chemical interaction between biocompatible polymers and ferric ions, and evaluate the survival, proliferation, and osteogenic differentiation of cells encapsulated in the hydrogels. The injectable hydrogels were prepared by simply mixing mixed alginate/gelatin solution at various ratios and FeCl3 solution. The hydrogels prepared disappeared within a few days in the phosphate buffered-saline solution (PBS) with containing collagenase although the disappearance rate increased with an increase of the gelatin ratio in the hydrogel. For the hydrogel of alginate/gelatin low ratio, the survival and proliferation of cells in the hydrogel-encapsulated condition were significantly high compared with those of hydrogel at the higher ratios. The cells collected 3 days after cultured in the hydrogel also proliferated to a significantly higher extent than those collected from other hydrogels. The proliferation ability of cells was similar that of cells cultured on the standard tissue culture polystyrene (TCPS) dish. When evaluated to compare with cells cultured on the TCPS dish, the expression of runt-related transcription factor-2 (RUNX2) gene, the alkaline phosphatase (ALP) activity, and the calcium precipitation were significantly high. The cells were encapsulated by the mixed alginate/gelatin and FeCl3 hydrogel and injected in the back subcutis of mice, the percentage of cells retained in the injected site was higher than that of cells injected in the PBS suspension. It is concluded that the injectable hydrogel prepared by simple mixing mixed alginate/gelatin solution and FeCl3 solution is a promising material for the cell transplantation.

Keywords: gelatin, alginate, physico-chemical interaction, injectable hydrogel

Suggested Citation

Anamizu, Mina and Tabata, Yasuhiko, Design of Injectable Hydrogels of Gelatin and Alginate with Ferric Ions for Cell Transplantation (July 21, 2019). Available at SSRN: https://ssrn.com/abstract=3423499 or http://dx.doi.org/10.2139/ssrn.3423499

Mina Anamizu (Contact Author)

Kyoto University - Laboratory of Biomaterials

Japan

Yasuhiko Tabata

Kyoto University - Laboratory of Biomaterials ( email )

Japan

Do you have negative results from your research you’d like to share?

Paper statistics

Downloads
84
Abstract Views
434
PlumX Metrics