Download This Paper
Preprints with The Lancet is part of SSRN´s First Look, a place where journals identify content of interest prior to publication. Authors have opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early stage research papers that have not been peer-reviewed. The findings should not be used for clinical or public health decision making and should not be presented to a lay audience without highlighting that they are preliminary and have not been peer-reviewed. For more information on this collaboration, see the comments published in The Lancet about the trial period, and our decision to make this a permanent offering, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com.
CD103 +CD4 + T Cell with Pro-Inflammatory Role Predominates in Helicobacter pylori- Positive Children Gastritis
35 Pages Posted: 15 Aug 2019
More...Abstract
Background: The mucosal resident T cells have been characterized in gastric tissue, but their role in Helicobacter pylori (H. pylori) infection children remains unclear.
Methods: Biopsy specimens were obtained from the gastric mucosa of H. pylori-positive children. Surface receptors, intracellular cytokines and transcription factors were determined by flow cytometry and western blot. Immunofluorescence was used to analyze CD4 / CD103 co-localization and TUNEL positive cells in stomach section.
Findings: Integrin receptor CD103 is highly expressed in CD4+ T cells of gastric mucosa from H. pylori-positive children. Mucosal resident CD103+CD4+ T cells exhibited a memory phenotype and high expression of chemokines compared with CD103— CD4+ T cells. In vitro co-culture study demonstrated that H.pylori specific antigen CagA/ VacA-primed DC induced proliferation of CD103+CD4+ T cells, as well as IFN-γ, TNF and IL-17 production. Increased T-bet and Blimp1 levels were also observed in CD103+CD4+ T cells in H.pylori-positive children. CD103+CD4+ T cells were positively correlated with numbers of dead gastric cells.
Interpretation: Our results explored the function of CD103+ T cells in H.pylori-positive children, which may provide therapeutic target for treatment of gastritis.
Funding Statement: This work was supported by grants from National Natural Science Foundation of China (81770552, 81801571).
Declaration of Interests: The authors state they have no financial conflicts of interest.
Ethics Approval Statement: The study was approved by the Ethics Committee of Guangzhou Women and Children's Medical Center, Jinan University. Biopsy specimens from the H. pylori-positive children were collected from Guangzhou Women and Children's Medical Center (Guangzhou, China), and an informed written consent was obtained from participants and their parents prior to commencement of the study.
Keywords: Helicobacter pylori; CD103+CD4+ T cells; Mucosal residence; Inflammation
Suggested Citation: Suggested Citation