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Fluorescent Conjugated Polymer Nanovector for in Vivo Tracking and Regulating the Fate of Stem Cells for Restoring Infarcted Myocardium

38 Pages Posted: 15 Jan 2020 Publication Status: Accepted

See all articles by Guorui Jin

Guorui Jin

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Wenfang Li

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Fan Song

Air Force Medical University - Department of Chinese Material Medical and Natural Medicines

Jing Zhao

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Mengqi Wang

Xi'an Jiaotong University (XJTU) - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research

Qian Liu

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Guoyou Huang

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Feng Xu

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education; Xi'an Jiaotong University (XJTU) - Bioinspired Engineering and Biomechanics Center (BEBC)

Abstract

Stem cell therapy holds great promise for cardiac regeneration. However, the lake of controllability in stem cell fate after in vivo transplantation greatly restricts the therapeutic outcomes of stem cell therapy. MicroRNA transfection has emerged as a powerful tool to control stem cell fate for enhanced cardiac regeneration. However, the clinical translation of therapy based on gene-transfected stem cells remains challenging, due to the unknown in vivo behaviors of stem cells. Here, we developed a novel nano-platform (i.e., PFBT@miR-1-Tat NPs) that can achieve triggered release of microRNA-1 for promoting cardiac differentiation of mesenchymal stem cells (MSCs), and long-term tracking of transplanted MSCs through the bright and ultra-stable fluorescence of conjugated polymer poly(9,9-dioctylfluorene-alt-benzothiadiazole) (PFBT). We found that PFBT@miR-1-Tat NP-transfected MSC treatment significantly restore infarcted myocardium through promoting stem cell cardiac differentiation and integration with the in-situ cardiac tissues. While, MSCs without gene transfection improve the infarcted heart functions mainly through paracrine effect and blood vessel formation. The developed conjugated polymer nanovector would be a powerful tool in manipulating as well as revealing the fate of therapeutic cells in vivo, which is critical for optimizing the therapeutic route of gene and cell combined therapy and therefore for accelerating clinical translation.

Keywords: Conjugated polymer nanoparticle, gene therapy, cell tracking, cardiac regeneration, cell therapy

Suggested Citation

Jin, Guorui and Li, Wenfang and Song, Fan and Zhao, Jing and Wang, Mengqi and Liu, Qian and Huang, Guoyou and Xu, Feng, Fluorescent Conjugated Polymer Nanovector for in Vivo Tracking and Regulating the Fate of Stem Cells for Restoring Infarcted Myocardium. Available at SSRN: https://ssrn.com/abstract=3518868 or http://dx.doi.org/10.2139/ssrn.3518868

Guorui Jin (Contact Author)

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Xi’an, 710049
China

Wenfang Li

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Xi’an, 710049
China

Fan Song

Air Force Medical University - Department of Chinese Material Medical and Natural Medicines

Xi'an, 710032
China

Jing Zhao

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Xi’an, 710049
China

Mengqi Wang

Xi'an Jiaotong University (XJTU) - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research

Xi’an, 710049
China

Qian Liu

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Xi’an, 710049
China

Guoyou Huang

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education

Xi’an, 710049
China

Feng Xu

Xi'an Jiaotong University (XJTU) - Key Laboratory of Biomedical Information Engineering, Ministry of Education ( email )

Xi’an, 710049
China

Xi'an Jiaotong University (XJTU) - Bioinspired Engineering and Biomechanics Center (BEBC) ( email )

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