To Development and Validation Spectrophotometric Estimation of Vildagliptin Through Oxidative Coupling Reaction Using MBTH Reagent in Pharmaceutical Dosage Form
Posted: 13 Feb 2020
Date Written: February 5, 2020
Abstract
Scientifically estimation of Vildagliptin in bulk and pharmaceutical dosage forms by 3-methyl-2-bezothiazoline hydrazone reagent using longer wavelength give strong absorbance. The purpose of this analytical validation procedure is to validate using ICH guideline.3-methyl-2-bezothiazoline hydrazone reacts with ferric chloride in the presence of amine group, Resulting green coloured species scanned in uv-visible spectrophotometric with the range 400-800 nm. Linearity concentration ranges 10 to 50 μg/ml and it given absorbance at 620 nm, Correlation coefficient value 0.998, Recovery studies obtain within range of 98.41 to 99.75% at maximum absorbance 620 nm. This method can be successfully applied for the estimation of drug content in pharmaceutical formulations. Resulting Analytical Validate method suitable for its intended use.
Keywords: 3-methyl-2-benzothiazoline hydrazone, Vildagliptin, Ferric chloride spectrophotometric determination, ICH guidelines
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