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A Bacteriophytochrome Mediates Interplay between Light Sensing and the Second Messenger Cyclic Di-GMP to Control Bacterial Social Behaviors

99 Pages Posted: 11 Mar 2020 Publication Status: Published

See all articles by Raj Kumar Verma

Raj Kumar Verma

Centre for DNA Fingerprinting and Diagnostics

Anindya Biswas

Centre for DNA Fingerprinting and Diagnostics

Akanksha Kakkar

Centre for DNA Fingerprinting and Diagnostics

Binod Bihari Pradhan

Centre for DNA Fingerprinting and Diagnostics

Subhadeep Chatterjee

Centre for DNA Fingerprinting and Diagnostics

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Abstract

Bacteriophytochromes are the most abundant and ubiquitous light sensing receptors in bacteria and are involved in time-of-day behavior or responses. However, their biological and regulatory role in non-photosynthetic bacteria is poorly understood, and even less is known about how it regulates diverse cellular processes. Here we show that a bacteriophytochrome (XooBphP), from the plant pathogen Xanthomonas oryzae pv. oryzae, perceives light signal and transduces through its EAL-mediated phosphodiesterase activity, modulating the intracellular level of the ubiquitous bacterial second messenger c-di-GMP. We discover that the light mediated fine-tuning of the intracellular c-di-GMP levels by XooBphP regulate the production of virulence associated functions, iron metabolism and transition of sessile to free-swimming motile lifestyle, contributing to the host colonization and virulence. Altogether, our results reveal that XooBphP plays a crucial role in integrating photo-sensing with intracellular signaling to control pathogenic lifestyle and bacterial social behaviors.

Keywords: photo-sensing, intracellular signaling, Type II Secretion, iron metabolism, attachment, virulence, motility

Suggested Citation

Verma, Raj Kumar and Biswas, Anindya and Kakkar, Akanksha and Pradhan, Binod Bihari and Chatterjee, Subhadeep, A Bacteriophytochrome Mediates Interplay between Light Sensing and the Second Messenger Cyclic Di-GMP to Control Bacterial Social Behaviors. Available at SSRN: https://ssrn.com/abstract=3549536 or http://dx.doi.org/10.2139/ssrn.3549536
This version of the paper has not been formally peer reviewed.

Raj Kumar Verma

Centre for DNA Fingerprinting and Diagnostics

Nacharam
Hyderabad
India

Anindya Biswas

Centre for DNA Fingerprinting and Diagnostics

Nacharam
Hyderabad
India

Akanksha Kakkar

Centre for DNA Fingerprinting and Diagnostics

Nacharam
Hyderabad
India

Binod Bihari Pradhan

Centre for DNA Fingerprinting and Diagnostics

Nacharam
Hyderabad
India

Subhadeep Chatterjee (Contact Author)

Centre for DNA Fingerprinting and Diagnostics

Nacharam
Hyderabad
India

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