Protein–Protein Interaction Methods and Protein Phase Separation

Posted: 31 Jul 2020

See all articles by Castrense Savojardo

Castrense Savojardo

University of Bologna - Department of Pharmacy and Biotechnology

Pier Luigi Martelli

University of Bologna - Department of Pharmacy and Biotechnology

Rita Casadio

University of Bologna - Department of Pharmacy and Biotechnology

Date Written: July 2020

Abstract

In the last decade, newly developed experimental methods have made it possible to highlight that macromolecules in the cell milieu physically interact to support physiology. This has shifted the problem of protein–protein interaction from a microscopic, electron-density scale to a mesoscopic one. Further, nowadays there is increasing evidence that proteins in the nucleus and in the cytoplasm can aggregate in membraneless organelles for different physiological reasons. In this scenario, it is urgent to face the problem of biomolecule functional annotation with efficient computational methods, suited to extract knowledge from reliable data and transfer information across different domains of investigation. Here, we revise the present state of the art of our knowledge of protein–protein interaction and the computational methods that differently implement it. Furthermore, we explore experimental and computational features of a set of proteins involved in phase separation.

Suggested Citation

Savojardo, Castrense and Martelli, Pier Luigi and Casadio, Rita, Protein–Protein Interaction Methods and Protein Phase Separation (July 2020). Annual Review of Biomedical Data Science, Vol. 3, pp. 89-112, 2020, Available at SSRN: https://ssrn.com/abstract=3658949 or http://dx.doi.org/10.1146/annurev-biodatasci-011720-104428

Castrense Savojardo (Contact Author)

University of Bologna - Department of Pharmacy and Biotechnology ( email )

Italy

Pier Luigi Martelli

University of Bologna - Department of Pharmacy and Biotechnology

Italy

Rita Casadio

University of Bologna - Department of Pharmacy and Biotechnology ( email )

Italy

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