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NADPH Performs Mediated Electron Transfer in Cyanobacterial-Driven Bio-Photoelectrochemical Cells

30 Pages Posted: 13 Oct 2020 Publication Status: Published

See all articles by Yaniv Shlosberg

Yaniv Shlosberg

Technion-Israel Institute of Technology - Grand Technion Energy Program

Benjamin Eichenbaum

Technion-Israel Institute of Technology - Faculty of Biology

Tünde N. N. Tóth

Technion-Israel Institute of Technology - Grand Technion Energy Program

Guy Levin

Technion-Israel Institute of Technology - Faculty of Biology

Varda Liveanu

Technion-Israel Institute of Technology - Faculty of Biology

Gadi Schuster

Technion-Israel Institute of Technology - Grand Technion Energy Program

Noam Adir

Technion-Israel Institute of Technology - Grand Technion Energy Program

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Abstract

Previous studies have shown that live cyanobacteria can produce photocurrent in bio-photoelectrochemical cells (BPEC) that can be exploited for clean renewable energy production. Electron transfer from cyanobacteria to the electrochemical cell was proposed to be facilitated by small molecule(s) mediator(s) whose identity (or identities) remain unknown. Here, we elucidate the mechanism of electron transfer in the BPEC by identifying the major electron mediator as NADPH in three cyanobacterial species. We show that an increase in the concentration of NADPH secreted into the extracellular medium (ECM) is obtained by both illumination and activation of the BPEC. Elimination of NADPH in the ECM abrogates the photocurrent while addition of exogenous NADP+ significantly increases and prolongs the photocurrent production. NADP+ is thus the first non-toxic, water soluble electron mediator that can functionally link photosynthetic cells to an energy conversion system and may serve to improve the performance of future BPECs.

Keywords: Photosynthesis, solar energy conversion, cyanobacteria, electron transfer, fluorescence, Ferrodoxin-NADP reductase, chronoamperometry

Suggested Citation

Shlosberg, Yaniv and Eichenbaum, Benjamin and Tóth, Tünde N. N. and Levin, Guy and Liveanu, Varda and Schuster, Gadi and Adir, Noam, NADPH Performs Mediated Electron Transfer in Cyanobacterial-Driven Bio-Photoelectrochemical Cells. Available at SSRN: https://ssrn.com/abstract=3696777 or http://dx.doi.org/10.2139/ssrn.3696777
This version of the paper has not been formally peer reviewed.

Yaniv Shlosberg

Technion-Israel Institute of Technology - Grand Technion Energy Program ( email )

Israel

Benjamin Eichenbaum

Technion-Israel Institute of Technology - Faculty of Biology ( email )

Technion City
Haifa 32000, 32000
Israel

Tünde N. N. Tóth

Technion-Israel Institute of Technology - Grand Technion Energy Program ( email )

Israel

Guy Levin

Technion-Israel Institute of Technology - Faculty of Biology

Technion City
Haifa 32000, 32000
Israel

Varda Liveanu

Technion-Israel Institute of Technology - Faculty of Biology ( email )

Technion City
Haifa 32000, 32000
Israel

Gadi Schuster

Technion-Israel Institute of Technology - Grand Technion Energy Program ( email )

Israel

Noam Adir (Contact Author)

Technion-Israel Institute of Technology - Grand Technion Energy Program ( email )

Israel

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