Larvicidal Evaluation of Aedes aegypti and Antioxidant, Cytotoxic and Antimicrobial Potential of the Aqueous Acid Extract of Pseudoxandra cuspidata (ANNOACEAE)

Abstract

The Anonnaceae family has 2,500 species and 135 genera, which has about 14 representatives of species in Brazil, including Pseudoxandra cuspidata. The objective of this research was to study the aqueous-acid extract of P. cuspidata, evaluating its antioxidant, larvicidal, antimicrobial and cytotoxic potential. In the preliminary phytochemical analysis, there were positivity to organicacids and alkaloids. Inhibitory Activity of EAA was 65% against DPPH. The AAE presented to A. Salina nauplii at LC50% of 600.79 μg/mL for a periodof 24 hours. In relation to larvicidal activity to Aedes aegypti intermediates, AAE presented the LC50% in 24 Hours of 475.91 ppm and 290.73 ppm in 48 Hours. Regarding the antimicrobial action, the EAA presented 50 mg/mL MIC for P. Aeruginosa and MBC at 100 mg/mL, and for S. aureus it presented MIC of 50 mg/mL and did not present MBC. This research characterized the presence of alkaloids and organicacids present in the aqueousacid extract of P. cuspidata. The extract presented lowtoxicity in relation to the microcrustacean A. salina. It also had a relevant antioxidant potential (65% of Inhibition), larvicidalaction considered effective, antimicrobialaction for both P. Aeruginosa and S. Aureus even did not present bacterici dalaction.

Keywords: Pseudoxandra, DPPH, Artemia salina, Aedes aegypti